Early gene synthesis primarily focuses on uncoating the genome and the next step is genome replication which needs to occur for intermediate gene synthesis. This is followed by late gene synthesis and virion morphogenesis.
The fields of biological research that gene synthesis has been applied to are not the same as targeted mutagenesis. Its main is to study a gene’s function in agriculture through the increase in crop production. One of the main benefits served by gene synthesis is that it can request a specific sequence of DNA in text format up to 150 Kb in length. This text format is like a physical copy with relative ease and high accuracy.
The sequence received, as a result, is then amplified multiple times with a focus on complying with the customer’s request. The accuracy is meant to give the customer the permit to produce a mutation anywhere within a gene of interest specific to their research topics. The mutation can be done anywhere but you can even use
any type of mutation: frameshift, deamination, deletion, insertion, or nonsense mutation.
Also, the gene synthesis makes it easy to upregulate a gene of interest in order to cause an overexpression of the resulting phenotype. All of these mutations can easily be requested and inserted or can also be applied to the sequence of interest to better understand the genetics research such as evolution, or antibiotic resistance.
Not only these applications, but gene synthesis has also a wide range of uses in agriculture through genetic engineering. Gene synthesis is the basis of genetic engineering and it gives permission to create a resistance gene to a particular bacteria for example. You can place your order in the Gene Order Portal of Bio Basic.
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